A novel biosurfactant producing Kocuria rosea ABR6 as potential strain in oil sludge recovery and lubrication

Abstract Biosurfactants are amphiphilic molecules composed of a hydrophilic and hydrophobic moiety had the ability to penetrate into different phases reduce surface tension. This features caused oil recovery, lubrication facilities crude in pipeline. In current research Biosurfactant-producing strain was isolated from storage tanks Isfahan Oil Refining Company Iran, screened by expansion test, droplet collapse, tension reduction measurement. Hydrocarbon recovery sludge measured under constant conditions. The effect factoring biosource on pipelines investigated vitro. Also, optimization biosurfactant production conditions as single factor using Response Surface Method (RSM). best biosurfactant-producing bacterium identified Kocuria rosea ABR6, its sequence registered gene bank with access number MK100469. Chemical analysis proved that produced lipopeptide. 7% recovered petroleum obtained ABR6. speed transfer upgraded it could be said for certain distance time reduced 64 35 s. highest at pH 9, aeration rate 120 rpm 96 h after incubation. use biosurfactants ABR6 is recommended remove lubricate industry.